AzuraQuant Probe Fast qPCR Mix HiRox 500 Reactions / 5 x 1 mL

AzuraQuant Probe Fast qPCR Mix NoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan, Scorpions and molecular beacon probes. AzuraQuant Probe Fast qPCR Mix is comprised of Azura HS Taq DNA Polymerase and an optimized buffer chemistry providing robust real-time PCR and broad range detection for increased sensitivity, speed, and reproducibility. In addition, the 2x master-mix exhibits excellent sensitivity in complex multiplex reactions, in which multiple amplicons can be detected without a loss in efficiency. AzuraQuant Probe Fast qPCR Mix requires little if any optimization and can be used to quantify any DNA templates including cDNA, genomic DNA, and low copy number viral sequences while providing resistance to many PCR inhibitors. AzuraQuant Probe Fast qPCR Mix also combines rapid antibody-mediated hot-start activation and processive enzyme chemistry, making it compatible with both standard and fast instrument cycling programs.

• Probe-based detection of DNA and cDNA
• TaqMan gene expression analysis
• Two-Step RT-qPCR
• Multiplex qPCR
• Flexibility: Compatible with standard and fast cycling instruments and a wide range of cycling parameters
• Accurate Quantification and Specificity: Hot-start chemistry reduces primer-dimer formation and allows room-temperature assembly.
• Broad Compatibililty: High-performance amplification from any DNA template including complex GC-rich sequences.
• Superior Multiplex Capability: Advanced formulation provides unrivalled sensitivity in singleplex as well as complex multiplex assays