AzuraQuant™ cDNA Synthesis Kit 25 Reactions

The AzuraQuant cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends. The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm reverse transcriptase blended with RNase inhibitor. The kit was developed to provide consistent linearity across a wide range of input RNA, revealing accurate relative cDNA representation, regardless of gene abundance. HighTherm reverse transcriptase which provides exceptional thermostability is blended with a potent RNase inhibitor to ensure high integrity of Total RNA starting material. The AzuraQuant cDNA Synthesis Kit is supplied with a high-performance 5x buffer system including an optimized ratio of random hexamer primers and anchored oligo (dT) primers which delivers unbiased, efficient and sensitive cDNA synthesis. The AzuraQuant cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. The AzuraQuant cDNA Synthesis Kit provides an ideal chemistry for success with limited starting material, such as tissue biopsies.

• 2-step RT-qPCR
• 2-step RT-PCR
• Unbiased sensitive cDNA synthesis: Complete 5' to 3' RNA sequence representation from as little as 4 pg Total RNA
• Reproducible: HighTherm reverse transcriptase coupled with an optimized 5x Buffer system generates consistent, high-yield qPCR-ready cDNA.
• Broad dynamic range: Optimized for dilute and low-copy input RNA.